n1s1 rat hepatoma cells  (ATCC)

Journal: Biomaterials Research

Article Title: Metabolic Gadolinium Labeling of Clostridium novyi -Nontoxic for Magnetic Resonance Imaging Visualization of Spores and Germinated Bacteria

doi: 10.34133/bmr.0326

Figure Lengend Snippet: In vivo MRI tracking of Gd-spores in an orthotopic liver tumor model. (A) In vivo experimental schedule for N1S1 tumor implantation, intra-arterial administration of Gd-spores (10 8 /ml, prepared with 10 mM GdCl 3 ), and MRI. (B) Representative T1-weighted (T1W) and T2-weighted (T2W) MR images showing dynamic changes in tumor contrast following intra-arterial infusion of Gd-spores. (C) Tumor signal-to-noise ratio (SNR) and (D) contrast-to-noise ratio (CNR) on T1-weighted MR images up to 9 days ( n = 3). Data are presented as mean ± SD (* P < 0.05, *** P < 0.001, **** P < 0.0001).

Article Snippet: N1S1 rat hepatoma cells (ATCC CRL-1603) were cultured with Iscove’s Modified Dulbecco’s Medium, and 3 × 10 6 cells in 100 μl of phosphate-buffered saline (PBS) were inoculated in the left lateral lobe of the liver region of rats after the laparotomy.

Techniques: In Vivo, Tumor Implantation

Journal: World Journal of Hepatology

Article Title: Ultrasound imaging in orthotopic hepatocellular carcinoma models: Promise, practicality, and points for refinement

doi: 10.4254/wjh.v17.i12.115551

Figure Lengend Snippet: Representative high-frequency ultrasound–based monitoring of orthotopic hepatocellular carcinoma established using the rat hepatoma cell line (N1S1). Tumor volume measurements obtained by ultrasound demonstrate an excellent correlation with reference histological and volumetric assessments ( r = 0.998, P < 0.001; Devan et al ), underscoring the accuracy, reproducibility, and experimental utility of this imaging approach for preclinical tumor evaluation. CT: Computed tomography; HCC: Hepatocellular carcinoma; 3D: Three dimensional; 3R: Replacement, reduction, and refinement. Created in BioRender ( www.biorender.com ).

Article Snippet: A syngeneic orthotopic HCC model was established in male Sprague Dawley rats by subcapsular implantation of the rat hepatoma cell line (N1S1) hepatoma cells into the left lateral liver lobe.

Techniques: Imaging, Computed Tomography

Journal: Hepatic Oncology

Article Title: APT imaging of hepatocellular carcinoma signals an effective therapeutic response in advance of tumor shrinkage

doi: 10.1080/20450923.2024.2389031

Figure Lengend Snippet: Changes in APT signal intensity (as a percentage) for the N1S1 tumors following in LDL-OA nanoparticle treatment. (Upper panel) T 2 -weighted axial MRI (lower panel) APT imaged co-registered with T2 axial MRI. The ATP image masks are overlaid the liver and tumor region. (Insert upper) Change in the APT signal in liver and tumor tissue before, 24 and 72 h post LDL-OA treatment. (Insert lower) H&E stained section showing liver and tumor interface. White arrows indicate tumor. Scale bar = 100 um.

Article Snippet: N1S1 rat hepatoma cell line (ATCC, CRL-1603, VA, USA) and the human liver tumor cell line HepG2 were cultured in Dulbecco's Modified Eagle's Medium (Sigma, D6429) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin.

Techniques: Staining

Journal: Hepatic Oncology

Article Title: APT imaging of hepatocellular carcinoma signals an effective therapeutic response in advance of tumor shrinkage

doi: 10.1080/20450923.2024.2389031

Figure Lengend Snippet: Changes in APT signal intensity (as a percentage) for the N1S1 tumors following in LDL-DHA nanoparticle treatment. (Upper panel) T 2 -weighted axial MRI (lower panel) APT imaged co-registered with T2 axial MRI. The ATP image masks are overlaid the liver and tumor region. (Insert upper) Change in the APT signal in liver and tumor tissue before, 24 and 72 h post LDL-DHA treatment. (Insert lower) H&E stained section showing liver and tumor interface. White arrows indicate tumor. *** represents p < 0.001 difference between groups. Scale bar = 100 um.

Article Snippet: N1S1 rat hepatoma cell line (ATCC, CRL-1603, VA, USA) and the human liver tumor cell line HepG2 were cultured in Dulbecco's Modified Eagle's Medium (Sigma, D6429) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin.

Techniques: Staining